Dynamics of Pancreas-Derived Organoids

Samuel Randriamanantsoa will talk about the tremendous potential of organoids as in vitro platforms for studying the developmental dynamics of both healthy and cancerous tissues.

During research into pancreatic ductal adenocarcinoma (PDAC), a highly lethal disease, spheroid cultures yielded insight into the fundamental biological aspects of the tumors. However, these spheroid cultures did not morphologically match the complex structures observed in vivo. Therefore, the researchers generated organoids from a single primary murine PDAC cell embedded in a collagen matrix. These organoids self-organized into highly branched structures displaying a seamless lumen that connected terminal end buds, thus replicating in vivo PDAC architecture.

Samuel will present how they identified distinct morphogenesis phases and their dynamics through live imaging, chemical perturbations, sequencing, and biophysical modeling.

Speaker

Samuel Randriamanantsoa
Technische Universität München, Germany

Samuel studied engineering and biophysics at Télécom Physique and the University of Strasbourg in France. In 2019, he joined the laboratory of Prof. Andreas Bausch at the Technische Universität München as a PhD candidate investigating the spatiotemporal development dynamics of organoids. His main research interests are in the self-organization processes of living systems at any scale.